Plasmid as a cloning vector with neomycin and streptomycin resistance genes, its use and methods for plasmid preparation and use as well as vaertsorganismer containing the plasmid or the plasmid with the inserted foreign DNA

摘要

Plasmid conferring resistance to streptomycin (SmR) and neomycin (NeoR) upon its host (e.g. a Bacillus, Staphylococcus or Escherichia coli) and which after having taken up a foreign DNA fragment at one of its restriction sites with loss of its SmR or NeoR phenotype, is still capable of replicating and expressing genetic information in its host.