A NOVEL METHOD TO OBSERVE PLASMID DNA COMPACTION INSIDE GENE VECTORS BY FLUORESCENCE RESONANCE ENERGY TRANSFER

机译：通过荧光共振能量转移观察基因载体内质粒DNA的新方法

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SUMMARY By using double fluorescent labeled plasmid DNA, the formation of the gene vectors and the release of free DNA were observed by fluorescence resonance energy transfer. This method appeared to be a reliable and valuable procedure to evaluate the stability of vectors in physiological environment including serum.

机译：发明内容通过使用双荧光标记的质粒DNA，通过荧光共振能量转移观察了基因载体的形成和游离DNA的释放。该方法似乎是评估载体在包括血清在内的生理环境中稳定性的可靠且有价值的方法。

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《International symposium on controlled release of bioactive materials;Consumer diversified products conference》|2001年|p.19-20|共2页
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K. Itaka; A. Harada; H. Kawaguchi; K. Nakamura; K. Kataoka;
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中图分类基础医学;
关键词
DNA; fluorescence; stability; release; polymeric micelles; gene therapy;

机译：脱氧核糖核酸;荧光稳定性;释放;聚合胶束;基因治疗;

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1. 一种用高拷贝质粒载体制备BAC载体基本功能基因的新方法 [J] . 崔红玉, 王亚萍, 徐明举, 农业科学与技术（英文版） . 2008,第002期
2. Intranuclear fluorescence resonance energy transfer analysis of plasmid DNA decondensation from nonviral gene carriers [J] . Yu Matsumoto, Keiji Itaka, Tatsuya Yamasoba, The journal of gene medicine . 2009,第7期

机译：非病毒基因载体中质粒DNA缩合的核内荧光共振能量转移分析
3. In situ single cell observation by fluorescence resonance energy transfer reveals fast intra-cytoplasmic delivery and easy release of plasmid DNA complexed with linear polyethylenimine [J] . Keiji Itaka, Atsushi Harada, Yuichi Yamasaki, The journal of gene medicine . 2004,第1期

机译：通过荧光共振能量转移的原位单细胞观察揭示了胞浆内快速传递和与线性聚乙烯亚胺复合的质粒DNA易于释放
4. Template-directed dye-terminator incorporation (TDI) assay: a homogeneous DNA diagnostic method based on fluorescence resonance energy transfer. [J] . Chen X, Kwok PY Nucleic Acids Research . 1997,第2期

机译：模板指导的染料终止剂掺入（TDI）分析：基于荧光共振能量转移的同质DNA诊断方法。
5. FLUORESCENCE RESONANCE ENERGY TRANSFER BASED QUANTUM DOT-DNA CONJUGATE FOR SENSITIVE DETECTION OF EPATITIS B VIRUS DNA [C] . Wang Xiang, Wang Yi, Guo Qing C., . 2009

机译：基于荧光共振能量转移的量子点DNA共轭物，用于肝炎B病毒DNA的灵敏检测
6. Acceptor detected fluorescence resonance energy transfer for measurement up to 250 A and biophysical studies on core TATA binding protein-DNA complex. [D] . Delgadillo, Roberto F. 2009

机译：受体检测到荧光共振能量转移，可测量高达250 A的电流，并对核心TATA结合蛋白-DNA复合物进行生物物理研究。
7. Kinking of DNA and RNA helices by bulged nucleotides observed by fluorescence resonance energy transfer. [O] . C Gohlke, A I Murchie, D M Lilley, 1994

机译：通过荧光共振能量转移观察到的凸起核苷酸使DNA和RNA螺旋弯曲。
8. 1E1545 The rates of TnT-movement on thin filament observed by time-resolved fluorescence resonance energy transfer methods [O] . Y. Shitaka, C. Kimura, M. Miki 2002

机译：1E1545随着时间分辨荧光共振能量转移方法观察到薄灯丝上的TNT移动的速率

A NOVEL METHOD TO OBSERVE PLASMID DNA COMPACTION INSIDE GENE VECTORS BY FLUORESCENCE RESONANCE ENERGY TRANSFER

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