METHOD FOR INCREASING THE MILK YIELD OF MILK-COW AND PAIL PARTICULARLY FOR CARRYING OUT THE METHOD

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  机译： 一种通过饲料增加奶牛产奶量的方法，制备该饲料的方法和实施该方法的设备

• 4. A method for increasing the milk yield of dairy cows by means of a feed, a method for preparing the feed and an apparatus for carrying out said method [P] . 欧洲知识产权局专利： EP0148143A3 . 1987-04-08

  机译： 一种通过饲料提高奶牛产奶量的方法，制备该饲料的方法和实施该方法的设备

• 5. A method for increasing the milk yield of dairy cows by means of a feed, a method for preparing the feed and an apparatus for carrying out said method [P] . 欧洲知识产权局专利： EP0148143A2 . 1985-07-10

  机译： 一种通过饲料提高奶牛产奶量的方法，制备该饲料的方法和实施该方法的设备

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• 1. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 2. Milk Spoilage: Methods and Practices of Detecting Milk Quality [J] . Michael Lu ,Yvonne Shiau ,Jacklyn Wong . 食品与营养科学（英文） . 2013,第7期
• 3. 我国奶牛饲料产奶净能值测算方法的研究 [C] . 冯仰廉 ,周建民 ,张晓明 . 第三届全国反刍动物营养学术研讨会 . 2011
• 4. 稀土与两种植物精油的不同组合对奶牛瘤胃微生物蛋白产量、产奶性能和氮排泄的影响 [A] . 袁腾 . 2019