VACCINE AND SERUM FOR ENDOTOXIN ASSOCIATED DISEASE AND METHOD OF PREPARING SAME AS WELL AS TO METHODS OF IMMUNIZATION AND TREATMENT OF SUCH DISEASE AND TO A DETOXIFIED ENDOTOXIN AND BACTERIAL MUTANT

摘要

ABSTRACTA combination vaccine, with methods of prep-aration and treatment, for protection against Gramnegative bacterial diseases. The vaccine includesa killed suspension of a bacterial mutant from thetaxonomic family Enterobacteriaceae, a B-lymphocyteproliferating immune modulator, and a protein and lipidbinding adjuvant. A hyperimmune serum for treatingdiseased animals is prepared by vaccinating a donoranimal with the combination vaccine and then preparingclot serum containing antigen specific antibodies.The mutant is ATCC No. 53000 and is produced by ionizingradiation of Salmonella enteritidis. The immune modula-tor is a detoxified extract of lipopolysaccharide andis useful with many antigens to enhance primary immuneresponse.