Cloning vectors comprising a restriction site bank and the construction thereof

摘要

Vectors designed for cloning any foreign DNA material in prokaryotic and eukaryotic organisms are provided, which vectors comprise a restriction site bank containing a large number of unique restriction sites. These vectors allow cloning of large DNA fragments and complete genes without the risk of unwanted cleavage of the DNA material by restriction enzymes. Described are also methods for preparing the vectors and processes using same for the cloning of DNA material, for the production of polypeptides etc.