Cloning of Ds MAPK Gene and Construction of Antisense Expression Vector

摘要

cqvip:In order to investigate the role of MAPK gene in adaptation of Dunaliella salina to hypersaline environment,the Ds MAPK gene of D. salina was amplified by PCR. After inverted insertion of the open reading frame of Ds MAPK gene into downstream sequence of 35 S promoter of plant expression vector,an antisense expression vector of Ds MAPK gene was successfully constructed and introduced into D. salina cells by LiA c/PEG-mediated method. The expression of Ds MAPK gene in transgenic D. salina was analyzed by semi-quantitative RT-PCR method. The results showed that the expression of Ds MAPK gene in D. salina was significantly inhibited at the transcriptional level. The study laid the foundation for further identification of the function of Ds MAPK gene.