Method for promoting intramolecular disulfide bond formation in recombinant proteins contained in a denaturant solution

摘要

A method is provided for promoting intramolecular disulfide bond formation in reduced recombinant proteins contained in a denaturant solution. Recombinant proteins which have been solubilized using a denaturant are reacted with an amount of the oxidizing agent cystine sufficient to oxidize free sulfhydryl groups and form intramolecular disulfide bonds.