Disulfide Bonds in a Recombinant Protein Modeled After a Core Repeat in anAquatic Insect's Silk Protein

摘要

We constructed a gene encoding rCAS, recombinant constant and subrepeat protein,modeled after tandem repeats found in the major silk proteins synthesized by aquatic larvae of the midge, Chironoinus ten tans. Bacterially synthesized rCAS was purified to near homogeneity and characterized by several biochemical and biophysical methods including amino-terminal sequencing, amino acid compositional