A^1HNMR STUDY ON THE FORMATION AND BREAKING OF INTRAMOLECULAR HYDROGEN BONDS OF BILIRUBIN IN CDCl_3-DMSO-d_6 BINARY SOLVENT

摘要

The formation and breaking of intramolecular hydrogen bondsof bilirubin in CDCl3-DMSO-d6 binary solvent have been investigated by meansof NMR spectroscopy.The chemical shifts of protons at dipyrrinone lactam C=Oand N-H,Pyrrole N-H,C-5,C-15 and methylene groups of 8,12-propionic acidside-chains changed markedly as a function of composition of the binarysolvent.The hydrogen bond formation is dependent on the conformation ofpropionic acid side-chains.