Monoclonal antibody against human manganese-superoxide dismutase, method for producing the monoclonal antibody, assay reagent or assay kit and assay method by use thereof, and diagnostic method of human ovarian cancer and myocardial infarction by use thereof

摘要

A monoclonal antibody against human manganese-superoxide dismutase characterized in that it is produced by a cell line which has been obtained by immunization of a mouse with human Mn-SOD and then fusion of lymphocytes obtained from the mouse with mouse myeloma cells, and has high specificity against human Mn-SOD; a method for producing a monoclonal antibody against human Mn-SOD having high specificity against human Mn-SOD, which comprises culturing the cell line obtained by immunization of a mouse with human Mn-SOD and then cell fusion of lymphocytes obtained from the mouse with mouse myeloma cells; a kit for assaying human Mn-SOD, comprising essentially:(a) a monoclonal antibody having very high specific immuno-reactivity against human Mn-SOD; and(b) a reagent of a monoclonal antibody having very high specific immuno-reactivity against human Mn-SOD labelled with an enzyme (enzyme labelled antibody); a method for assaying human Mn-SOD by use of the kit; a novel diagnostic method of human epithelial ovarian cancer, which comprises assaying the concentration of human Mn-SOD in a body fluid by use of the assay kit; and a novel diagnostic method of myocardial infarction, which comprises assaying the concentration of human Mn-SOD in a body fluid by use of the assay kit.