P. Falciparum in human blood samples and in mosquito extract, which uses a single plate pretreated with only the synthetic antigen (NANP)₂₀ using, as total competitive inhibitor for the formation of the complex between the synthetic antigen adsorbed on the plate and the antibody contained in the sample, the (NANP)₂₀ synthetic peptide in a weight ratio of at least 20:1 with respect to the adsorbed antigen.;Because of its high specificity, sensitivity and speed, said method is particularly suitable for epidemiological studies on malaria."/> Immunoenzymatic single-plate elisa method with competitive inhibitition for detecting antisporozoite antibodies of plasmodium falciparum
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Immunoenzymatic single-plate elisa method with competitive inhibitition for detecting antisporozoite antibodies of plasmodium falciparum

机译:具有竞争抑制力的免疫酶联单板酶联免疫吸附法检测恶性疟原虫的抗子孢子抗体

摘要

An ELISA method with competitive inhibition is described for determining antisporozoite antibodies of P. Falciparum in human blood samples and in mosquito extract, which uses a single plate pretreated with only the synthetic antigen (NANP)₂₀ using, as total competitive inhibitor for the formation of the complex between the synthetic antigen adsorbed on the plate and the antibody contained in the sample, the (NANP)₂₀ synthetic peptide in a weight ratio of at least 20:1 with respect to the adsorbed antigen.;Because of its high specificity, sensitivity and speed, said method is particularly suitable for epidemiological studies on malaria.
机译:描述了一种具有竞争性抑制作用的ELISA方法,用于测定 P的抗子孢子抗体。人体血液样品和蚊子提取物中的恶性疟原虫,使用仅用合成抗原(NANP)2预处理的单板,用作总竞争性抑制剂,以形成吸附在该板和样品中所含的抗体(相对于吸附抗原的重量比至少为20:1的(NANP)20合成肽)。由于其特异性高,灵敏度高和速度快,该方法特别适用于流行病学研究关于疟疾。

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