METHOD FOR PURIFICATION OF PRE-S2 BY IMMUNO AFFINITY CHROMATO GRAPHY

摘要

A method for purifying pre-S2 comprises (a) pregp. crude sample of recombined amino (and carboxy) terminal-pre-S2- betagalactosydase from colon bacteria, (b) cutting pre-S2 chain by treatment of CNBr, and (c) sepg. pre-S2-peptide by 6-aminophenyl1-thio-beta- galactopyranosyl-CH-sepharose 4B (TPEG-Sepharose 4B) affinity chromatography.