PREGNANCY TEST APPARATUS USING IMMUNO CHROMATO GRAPHY

摘要

The present invention provides a diagnostic kit using solid phase immunochromatography.;The diagnostic device according to the present invention is a band in which an anti-chlea gonadotropin-monoclonal antibody (anti-HCG-MAb-1) is a fixed band, and a band in which an anti-rat IgG polyclonal antibody is fixed. A larvae solid phase (4) equipped with a reaction terminator (6), an anti-villi gonadotropin-monoclonal antibody (anti-HCG-MAb-2) that binds to an antigen at a site different from the anti-HCG-MAb-1. ) Latex drop membrane (2) containing colored latex particles bound together, and spread from the latex drop membrane (2) to the caterpillar solid phase (4) in the resulting readout line (5) and the reaction termination line (6). Pregnancy diagnosis device by solid-state immunochromatography with result reading window (7), reaction completion window (8), absorbent paper (3), (9) and absorbent rod (1) for color development (4) is a nitrocellulose membrane having a pore size of 15 to 25 times the size of colored latex particles, and 0.1 to 1 casein as a blocking agent. %, Bovine serum albumin 0.05 ~ 5% and borate buffer solution (pH 7.0 ~ 9.0) characterized in that it contains a mixture of 0.05 ~ 0.5M.;The device of the present invention can be used for the diagnosis of pregnancy by using monoclonal antibodies against chorionic gonadotropin, in particular, as an antibody, which makes it possible to determine whether pregnancy is faster and more accurate than conventional devices.