Silica-supported Macroporous Chitosan Bead for Affinity Purification of Trypsin Inhibitor

摘要

Macroporous cross-linking chitosan layer coated on silica gel (CTS-SiO2) was prepared by phase inversion and polyethylene glycol (PEG) molecular imprinting methods. Formation of macroporous surface was investigated by scanning electron microscopy (SEM) and BET analysis. The prepared bead was activated by reacting with 1,2-ethylene diglycidyl ether for introducing epoxy groups, and trypsin could be efficiently immobilized on the bead as a biospecific ligand. The bead bearing trypsin was employed to purify trypsin inhibitor (TIs) from egg white as affinity adsorbent.