Biologically reactive particles with biological, therapeutic and chromatographic applications

摘要

The particles comprise (a) a metal oxide/hydroxide core to which is bound (b) functionalized organophosphorus compound such that the P-containing groups are bound to the active sites of the core and which forms a monomolocular layer with the functionalized C-containing groups oriented away from the core surface and (c) biologically active ligand bound to the exposed functional groups. The ligand may comprise a whole cell or an antibody, antigen, hapten, enzyme particularly protein A) or substrate or inhibitor thereof, cofactor, binding or carrier protein or compound bound thereto, hormone, receptor, repressor, inducer, oligonucleotide, nucleic acid, amino acid, saccharide, dye (particularly Cibacron Blue), polyacrylamide, latex or ion-exchange material. The particles are used to immobilise enzymes or single strand DNA/RNA, in cell sorting and immunoassay, in the treatment of disease, and or packing material in chromatographic methods.