QUICK IDENTIFICATION OF MYCOBACTERIUM TUBERCULOSIS

摘要

PURPOSE:To specifically identify Mycobacterium tuberculosis in high reproducibility in a short time by amplifying the specific objective base sequence of Mycobacterium tuberculosis using PCR(polymerase chain reaction) process and detecting and identifying the amplified sequence. CONSTITUTION:The base sequence of formula I, etc., and the base sequence of formula II, etc., are used as a sense primer and an antisense primer, respectively. These primers are added together with a DNA polymerase to a 10mM tris-HCl buffer solution of pH8.3. A sample DNA separated from Mycobacterium tuberculosis is added to the solution and subjected to 30 cycles of reaction with a thermal cycler. One cycle of the reaction consists of a reaction at 94 deg.C for 30sec, a reaction at 55 deg.C for 30sec and a reaction at 72 deg.C for 1min. The specific objective base sequence of Mycobacterium tuberculosis amplified by the above PCR process is detected and identified by conventional process to enable the quick and sensitive identification of Mycobacterium tuberculosis.