Quick Detection of Mycobacterium tuberculosis Bacteria on Suspected Extrapulmonary Tuberculosis Patients Using MGIT960 Liquid Media Culture

摘要

Extrapulmonary tuberculosis (extrapulmonary tuberculosis/EPTB) is an infectious disease caused by the bacterial Mycobacterium tuberculosis (M. tuberculosis) in organs other than the lungs, including pleura, lymph nodes, the lining of the brain, pericardium, bones, joints, skin, intestines, kidneys, channels pee and so on. The diagnosis of supporting tuberculosis is very difficult because there are very few M tuberculosis bacterial cells in the EPTB specimen. The study of patients suspected of tuberculosis using the MGIT960 culture method aims to detect M. tuberculosis bacterial quickly and accurately compared to AFB staining and Lowenstein Jensen culture. A total of 32 specimens consisting of liquor cerebrospinalis, effusion pleural and pus in the bone were carried out AFB staining, MGIT960 and Lowentein Jensen cultures which were identified using MPT64 rapid test, found M tuberculosis in liquor cerebrospinal 2 (6.25%), pus in bone 1 (3.2 %) and Mycobacterium Other Than Tuberculosis (MOTT) in effusion pleural 2 (6.25%) Conclusions MGIT960 culture can detect M tuberculosis in EPTB patients