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MODIFIED AMINO-ACID SENSITIVE IMMOBILIZED ERYTHROCYTE, MANUFACTURE THEREOF AND DETECTING METHOD FOR ANTIGEN OR ANTIBODY USING THIS ERYTHROCYTE
MODIFIED AMINO-ACID SENSITIVE IMMOBILIZED ERYTHROCYTE, MANUFACTURE THEREOF AND DETECTING METHOD FOR ANTIGEN OR ANTIBODY USING THIS ERYTHROCYTE
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机译:修饰的氨基酸敏感型固定化红细胞,其制备方法以及使用该红细胞的抗原或抗体的检测方法
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摘要
PURPOSE:To establish a determing method which indicates high potency simply and is suitable as a screening method by adding modified amino acid to an erythroycte suspension, thereafter adding alhehyde, and performing the immobilization of the erythrocytes and the sensitization of the modified amino acid on an erythrocyte film at the same time. CONSTITUTION:Modified amino acid is added into the erythrocyte suspension. Then aldehyde is added. The immobilization of the erythrocytes and the sensitization of the modified amino acid to an erythrocyte film are performed at the same time. As the aldehydes, glutaraldehyde, formaldehyde and the like can be listed. The glutaraldehydfe is preferable since it indcates excellet reactivity. As the modified amino acid, the amino acids which are modified by phosphorylation, sulfonation, acylation and the like, e.g. tyrosine phosphate and the like, are used. The tyrosine phosphate is especially important in the sensor for detecting an anti-tyrosine phosphate antibody that is most effective for observing the kinetics of tyrosine phosphorylation. Thus, the immobiliztion and the sensitization of the erythrocyle which must have been individually conducted can performed at the same time. Therefore, the sensitive fixed erythrocyte indicating excellent activity can be simply obtained.
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