TRANSFORMED BACTERIUM PSEUDOMONAS PUTIDA KF13 STRAIN HAVING BIPHENYL METABOLIZING FUNCTION

摘要

PURPOSE:To obtain a germicide to a specific mold, by recombining a specific adventitious gene with vector PKF330 derived from Escherichia coli and transforming the recombinant plasmid PMFB1 into Pseudomonas putida. CONSTITUTION:An adventitious gene derived from Pseudomonas pseudo alcaligenes, having biphenyl metabolizing function, 7.9 kilo base molecular weight and number of base cleavage characterized by restriction enzymes shown by the formula I is recombined with vector PKF330 derived from Escherichia coli. The recombinant plasmid PMFB1 is transformed into host bacterium Pseudomonas putida, to give transformed bacterium Pseudomonas putida KF138 strain having biphenyl metabolizing function.