MANUFACTURE OF IMMOBILIZED GLUCOSE OXIDATION ENZYME MEMBRANE BY PLASMA SURFACE TREATING PROCESS

摘要

PURPOSE: To produce an active and stable membrane by treating a hydrophilic membrane with a low temperature plasma generated in a specific condition, then immersing the treated membrane into an aqueous 1,5-glutaraldehyde solution and then into an aqueous glucose oxidase solution. ;CONSTITUTION: This method for producing an immobilized glucose oxidase membrane is to place a hydrophilic polypropylene(PP) membrane on an electrode 11 of a plasma reaction vessel 10 in the figure, introducing an inert gas such as argon and ammonia into a reaction chamber 12, maintain the pressure in the reaction chamber 12 by adjusting a needle valve of a flow meter 30, treating the hydrophilic PP membrane for ≤200 sec with a low temperature plasma generated by 40-100 W irradiating discharge at 13.56 MHz radiowave frequency of the irradiating discharge under 0.4-1.0 Torr pressure inert gas atmosphere, then immersing the treated membrane into an aqueous 1,5-glutaraldehyde solution, and then into an aqueous glucose oxidase solution. Thereby, the obtained immobilized glucose oxidase membrane can be used in the production of a biological sensor, and the obtained biological electrode is very active and also stable.;COPYRIGHT: (C)1991,JPO