首页> 外国专利> A METHOD FOR SIMULTANEOUS DETECTION OF DIFFERENT TYPES OF ANTIBODIES AND/OR ANTIGENS PRODUCED BY INDIVIDUAL CELLS

A METHOD FOR SIMULTANEOUS DETECTION OF DIFFERENT TYPES OF ANTIBODIES AND/OR ANTIGENS PRODUCED BY INDIVIDUAL CELLS

机译:同时检测单个细胞产生的不同类型抗原和/或抗原的方法

摘要

In the process described, which is used to detect antibodies and / or antigens secreted and / or released by individual cells, a cell suspension is contacted with a solid support. After or during this contacting step, adding at least two different types of other antibodies directed against respectively the antibodies or antigens to be detected and having the ability to bind respectively to these antibodies or to these antigens to be detected, these other antibody being further provided with different enzymes. was then added the corresponding number of indicator substances, which have a different coloring effect for the simultaneous detection of different types of antibodies and / or antigens by evaluating the distinct color spots remaining on the solid support. According to the described embodiment, two solid supports are used, the opposite surfaces of which are attached antigens and / or antibodies of the same type or a different type, the cell suspension being applied between these surfaces.
机译:在所述的用于检测单个细胞分泌和/或释放的抗体和/或抗原的方法中,使细胞悬浮液与固体支持物接触。在该接触步骤之后或期间,添加至少两种不同类型的其他抗体,分别针对待检测的抗体或抗原,并具有分别与这些抗体或与待检测的抗原结合的能力,进一步提供了这些其他抗体与不同的酶。然后加入相应数量的指示剂物质,这些指示剂物质具有不同的着色效果,可通过评估固相支持物上保留的不同色斑来同时检测不同类型的抗体和/或抗原。根据所描述的实施方案,使用两个固体支持物,其相对表面附着有相同类型或不同类型的抗原和/或抗体,在这些表面之间施加细胞悬浮液。

著录项

相似文献

  • 专利
  • 外文文献
  • 中文文献
获取专利

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号