STRAIN OF CULTURED HYBRID ANIMAL CELLS MUS MUSCULUS L PRODUCING MONOCLONAL ANTIBODIES AGAINST HUMAN ALPHA-PHETOPROTEIN

摘要

the invention u043eu0442u043du043eu0441u0438u0442u0441u00a0 to u0433u0438u0431u0440u0438 - u0434u043eu043cu043du043eu0439 u0442u0435u0445u043du043eu043fu043eu0433u0438u043d and may be used u0434u043bu00a0 u043eu043fu0440u0435u0434u0435u043bu0435u043du0438u00a0 alpha u0444u0435u0442u043eu043fu0440u043eu0442u0435u0438u043du0430 (u0430u0444u0438) u0438u043cu043cu0443u043du043eu0440u0430u0434u0438u043eu043cu0435u0442u0440u0438 economic method. purpose to obtain a hybrid u0438u0437u043eu0431u0440u0435u0442u0435u043du0438u00a0 strain cultured cells of animals Mus inusculus l., producing monoclonal antibodies (u043cu043eu043du0430u0442 afp) to humans and animals. u044bu0442u0430u043cu043c receive u0433u0438u0431u0440u0438u0434u0438u0437u0430u0446u0438u0435u0439 u044563 myeloma cells.653 with u0441u043fu043bu0435u043du043e - u0446u0438u0442u0430u043cu0438 mice u0432u0430u0435u0432 / c, u0438u043cu043cu0443u043du0438u0437u0438u0440u043eu0432u0430u043d - u043du044cu0433u0445 afp achieve integrity rights, the strain is deposited with the u0432u0441u043au043a (ii) no. 346.D. strain produced u043cu043eu043du0430u0442 class IftGI. cells with the environment DMEM or RPMT 1640 with 15% fetal cattle serum and standard additives, with 5% u044102 in air. u043fu0435u0440u0435u0432u0438u0432u0430u044eu0442 cells in vivo after 3 - 4 u0434u043du00a0 with initial density (1 - 2).cells of ra's body in the form of u0430u0441u0446u0438u0442u043du044bu0445 u0441u043du043du0433u0435u043du0446u0438u0445 mice tumors. titer u043cu043eu043du0430u0442 in u0430u0441u0446u0438u0442u043du044bu0445 u0436u0438u0434u043au043eu0441u0442u00a0u0445 10 when determining method EITSA. s