PHAGE DISPLACEMENT VECTOR K15 FOR CONSTRUCTING OF STRUCTURE GENES LIBRARY FOR DNA

摘要

A substitution phage vector lambda SK15 for construction of kDNA structure gene libraries along BamI and EcoRI restrictase identification sections has the size of 51.2 k.b.p of nucleotides, the vector capacity of 0.2 to 15.4 k.b.p of nucleotides. lambda SK15 consists of: DNA of phage lambda EMBL3; DNA of plasmid SK2 DELTA SR; a kanamycin resistant gene of plasmid pUC4K; Hind III-fragment of DNA phage lambda of a size of 2.0 k.b.p of nucleotides. The host spectrum for the strain E. coli: LE392 = F', hsd R514 (rk, mk), sup E44, sup F58, lac YIOZ (lac IZY)6, galK2, galT22, metB1, trpR55, j.M.109 = rec DELTA I, DELTA Iac pro, end A1, gyr A96, thi-1, hsd R17, sup. E44, rel A1, F', tra D36, pro AB+, lac IqZ DELTA M15. Q359 = hsdRk-, hsd Mk+, sup F, O 80, P2. The claimed vector was deposited on 21.07.89 at the all-Union Collection of Industrial Microorganisms of the USSR Research Institute for Genetics and Industrial Microorganism Breeding and was registered under number PH No 688.