Recombinant plasmids for pox virus transformation - contg. multifunctional DNA sequence cassette to facilitate cloning of foreign DNA

摘要

Recombinant plasmids contain at least one replicon, at least one selectable marker, at least one promoter with flanking regions derived from a pox virus genome, and a multifunctional DNA sequence cassette (I) following the promoter. Also claimed are recombinant plasmids as above contg. a gene coding region insert, recombinant pox viruses contg. such plasmids, the use of such viruses to express foreign DNA sequences, cell cultures infected with such viruses, (poly) peptides produced by such cell cultures, medicaments, vaccines and diagnostic agents contg. such (poly)peptides, live vaccines contg. recombinant pox viruses as above, and the use of such vaccines to produce antibodies Pref. the specified plasmid is pSC11-Orth (DSM 5734), produced by linearising pSC11 (DSM 4381) with SmaI and inserting the multiple cloning site sequence of formula 5' GCA CAT ACG ATT TAG GCC TAG GAT GTC GAC TAG TTA GCT AGC GTA TTA ATT TCG ATC 3' (Ia) (where (Ia) has SteI, AvrII, SalI, AccI, HindII, SpeI and NheI cleavage sites). -