HYBRID PROTEINS BETWEEN AN EXTRACYTOPLASMIC ENZYME AND ANOTHER PROTEIN, PROCESS FOR THE PREPARATION THEREOF, AND THEIR APPLICATION AS DIAGNOSTIC AGENTS

摘要

Hybrid proteins between an extracytoplasmic enzyme and another protein, such as an antigen or a single Fv variable fragment of an antibody, their method of preparation, and their application as a diagnostic agent. / P P DNA sequences coding for said hybrid proteins, the recombinant plasmids containing said sequences, the expression vectors comprising said plasmids and the strains of bacteria or yeasts capable of being hosts of said plasmids and / or vectors are described as well as their application for the production and the secretion of said hybrid proteins. The protein consists of a hybrid sequence comprising at least one fragment of an enzyme exported or secreted by a microorganism chosen in particular from the group comprising the bacteria and yeasts and a suitable protein, whose free N-terminus and / or free C-terminus are not essential to its stru and the expression of some of its biological activities and possesses both the properties of the enzyme and certain properties of the protein, including the ability to interact specifically with an antibody or antigen. / P