DNA FOR PREPARING TRANSGENIC ANIMAL, FERTILIZED OVUM-INJECTING AGENT COMPRISING THE DNA, AND PREPARATION OF TRANSGENIC ANIMAL USING THE SAME

摘要

NEW MATERIAL:A linear DNA whose both ends have structures selected from structures of formulas I, II and III (the ... parts are base sequences containing genes to be introduced). USE:The preparation of transgenic animals, etc. PREPARATION:The DNA ends having the formulas I, II and/or III are prepared by treating DNA ends containing the objective genes with S1 unclease, etc., so as to have smooth ends, and subsequently combining the treated DNA ends with a linker having a preliminarily synthesized above-mentioned base sequence by the use of T4DNA ligase.