RECOMBINANT DNA STRUCTURE, RECOMBINANT VECTOR, METHOD FOR OBTAINING PROTEIN FROM MILK OF TRANSGENIC ANIMAL, METHOD FOR PRODUCING TRANSGENIC ANIMAL AND MILK OF TRANSGENIC ANIMAL

摘要

The invention relates to a recombinant DNA construct for the expression of proteins in the mammary gland of transgenic mammals and subsequent secretion into the milk, which contains the following DNA regions in the stated sequence and in functional linkage: (1) a transcription control region composed of one or more genes which are specifically activated in the mammary gland, (2) a first DNA sequence region which is not translated, (3) a DNA sequence which codes for a single peptide which permits secretion into the milk, (4) a DNA sequence which contains the genetic information for a heterologous peptide or a protein and (5) a DNA sequence which contains a polyadenylation site and a transcription termination signal. The invention further relates to a recombinant vector which contains a construct of this type, and to methods for obtaining proteins from the milk of transgenic animals in whose genome a DNA construct according to the invention is integrated.