METHOD FOR PROLIFERATING CALLUS DERIVED FROM STIGMA PART OF CROCUS SATIVUS L.

摘要

PURPOSE:To efficiently proliferate a callus derived from stigma of Crocus sativus L. of Iridaceae by using a specific culture medium. CONSTITUTION:A callus derived from a stigma part of Crocus sativus L. is inoculated into a culture medium containing 0.1 uM to 90mM inorganic ingredient (e.g. NH4NO3), 10-90g/l carbon source (e.g. saccharide), 0.1-100mg/l plant hormones (e.g. 1-naphthalene acetic acid), 0.1-1000mg/l vitamins (e.g. thiamine) and 0-1000mg amino acids (e.g. glycine) and being =30% in the ratio of nitrogen amount as ammonia contained in total nitrogen amount to proliferate the callus.