DNA AND RNA PROBE TO SIMULTANEOUSLY DETECT VIBRIO CHOLERATE AND ENTEROTOXIGENIC ESCHERICHIA COLI AND DETECTION OF VIBRIO CHOLERA AND ENTEROGEXIGENIC ESCHERICHIA COLI USING THE SAME DNA AND RNA PROBE

摘要

NEW MATERIAL:A probe prepared by linking toxicogenic genes LTH, STIa and STIb of enterotoxigenic Escherichia coli showing pathogenicity to human with T4DNA ligase. USE:For detecting Vibrio cholerae and enterotoxigenic Escherichia coli, etc. PREPARATION:Plasmid KAD007 is obtained from plasmid pKAD001 containing gene range of plasmid 1032H-19-del-EcoRI, STIa containing LTH gene range and plasmid pKAD002 containing STIb gene range, LTH-STIa-STIb trivalent prove fragment is inserted into XbaI-EcoRI site of BLUESCRIPT SK(-), the plasmid is made linear to give pKAD008, which is digested with XbaI to give the title probe.