RECOMBINANT PLASMID DNA P VR6-1 THE SIZE OF 3406 NUCLEOTIDE PAIRS USED FOR PREPARING SPECIFIC DNA-PROBE FOR HUMAN ROTAVIRUS 1-ST SUBGROUP DETECTION AND THE STRAIN OF TRANSFORMED MICROORGANISM ESCHERICHIA COLI USED FOR PREPARING RECOMBINANT PLASMID DNA P VR6-1 AND SPECIFIC PROBE FOR HUMAN ROTAVIRUS 1-ST SUBGROUP DETECTION

摘要

FIELD: medicinal biotechnology, virology. SUBSTANCE: invention relates to preparing recombinant plasmid DNA pVR6-1 used for probe isolation which is used for specific detection of human rotaviruses of the 1-st subgroup and preparing transformed bacteria Escherichia coli which contain plasmid DNA pVR6-1. Obtained plasmid has SmaI-SmaI-fragment of cDNA the size of 660 nucleotide pairs which shows homology with the site of the 6-th gene human rotaviruses of the 1-st subgroup. Probe synthesis level in plasmid in constructed strain E. coli is 1.5 mg/ml at culture density 5 x 10⁸ cells/ml. Plasmid can be used for viral diseases diagnosis in human. EFFECT: preparing plasmid indicated above. 3 cl, 4 dwg