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PROTEINS, PEPTIDES, DNA SEQUENCES, DNA OR RNA HYBRIDIZATION EVIDENCE, ANTIBODY, TUBERCULOSIS IMMUNE DIAGNOSIS, MICROORGANISM, TUBERCULOSIS VACCINE, PROCESS FOR TUBERCULOSIS TUBERCULOSIS TUBERCULOSIS
PROTEINS, PEPTIDES, DNA SEQUENCES, DNA OR RNA HYBRIDIZATION EVIDENCE, ANTIBODY, TUBERCULOSIS IMMUNE DIAGNOSIS, MICROORGANISM, TUBERCULOSIS VACCINE, PROCESS FOR TUBERCULOSIS TUBERCULOSIS TUBERCULOSIS
Disclosed are the immunochemical properties of a 17 kDa protein antigen fractionated, purified and sequenced for amino acids from Mycobacterium tuberculosis (South Indian isolate, SII 1) which causes human tuberculosis worldwide among 16 million patients. The 17 kDa protein antigen which has an N-terminus of A T T L P V Q R (aa 1-8) has at least three specific antibody binding epitopes located on linear peptides of sequences, R A T Y D K R Y E V R (aa 91-101) and S E F A Y G S F V R (aa 68-77) which were useful in a micro ELISA for the early diagnosis of human tuberculosis by the detection of specific antibodies. The 17 kDa protein antigen which was mitogenic for human tuberculous peripheral blood lymphocytes was found to carry three predicted T-cell epitopes on linear peptides of sequences, S E F A Y G S F V R (aa 68-77) and A E L P G V D P D C D V C I T R (aa 107-122). The 17 kDa antigen of M. tuberculosis (SII 1) which thus had both B and T cell reactive properties was found to contain 131 amino acids and potentially applicable in the immuno diagnosis, immuno therapy and immuno prophylaxis of human tuberculosis.
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机译:公开了对来自结核分枝杆菌(南印度分离株,SII 1)的氨基酸进行分馏,纯化和测序的17kDa蛋白抗原的免疫化学性质,其导致全世界1600万患者中的人类结核病。具有ATTLPVQR(aa 1-8)N端的17 kDa蛋白抗原具有至少三个位于序列线性肽上的特异性抗体结合表位,分别是RATYDKRYEVR(aa 91-101)和SEFAYGSFVR(aa 68-77),可用于微ELISA中,通过检测特异性抗体对人结核病进行早期诊断。发现对人结核性外周血淋巴细胞有丝分裂的17 kDa蛋白抗原在序列的线性肽S E F A Y G S F V R(aa 68-77)和A E L P G V D P D C D V C I T R(aa 107-122)上带有三个预测的T细胞表位。结核分枝杆菌(SII 1)的17 kDa抗原同时具有B细胞和T细胞的反应性,被发现含有131个氨基酸,可能适用于人类结核病的免疫诊断,免疫治疗和免疫预防。
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