METHOD OF SEMI-QUANTITATIVE DETERMINATION OF PHOSPHORUS CONTENT IN THE URINE OF CATTLE, PARTICULARLY COWS

摘要

After ca 10 minutes and frequent stirring of a mixture of 1 part of a solution collected from a freshly prepared mix of undiluted analysed urine, 2M trichloro acetic acid and distilled water in a volume ratio of 4:1:1, two parts of a reagent are added. The reagent is prepared immediately before use by mixing equal parts of a 2 mM aqueous solution of ammonium vanadate and a 40 mM aqueous solution of ammonium molybdate. The reaction mix is homogenised by stirring and after 15 minutes its resulting colour is assessed visually.IMAGE