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METHOD OF AMPLIFYING TARGET NUCLEOTIDE SEQUENCE AND DETECTION OF ALLELES INDICATIVE OF AN INHERITED DISEASE OR TRAIT
METHOD OF AMPLIFYING TARGET NUCLEOTIDE SEQUENCE AND DETECTION OF ALLELES INDICATIVE OF AN INHERITED DISEASE OR TRAIT
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机译:扩增目标核苷酸序列并检测遗传性疾病或性状的等位基因的方法
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摘要
A method for the amplification of a target nucleotide sequence using a first primer comprising 1) a target binding nucleotide moeity which is substantially complementary to the desired portion of the target nucleotide sequence; and 2) a polynucleotide tail; the first primer being such that whilst an extension product thereof may itself serve as a template for primer extension to form an amplification primer extension product, formation of an amplification primer extension product comprising a sequence complementary to the polynucleotide tail is inhibited.
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