device and method for fumigation of fluessigkeiten boundary, especially of culture media for propagation of tissue culture.

• 1. 用于胭脂萝卜组织培养的MS培养基、丛生芽培养基及胭脂萝卜的组织培养快速繁殖方法 [P] . 中国专利： CN106550875B . 2018.09.28
• 2. 用于胭脂萝卜组织培养的MS培养基、丛生芽培养基及胭脂萝卜的组织培养快速繁殖方法 [P] . 中国专利： CN106550875A . 2017-04-05
• 3. device and method for fumigation of fluessigkeiten boundary, especially of culture media for propagation of tissue culture. [P] . 德国专利： DE3685579D1 . 1992-07-09

  机译： 用于熏蒸氟西酮边界，尤其是用于组织培养繁殖的培养基的熏蒸的装置和方法。

• 4. method and device for fumigation of fluessigkeiten boundary, especially of culture media for propagation of tissue culture. [P] . AT48155T . 1989-12-15

  机译： 熏制氟西酮边界的方法和装置，特别是用于组织培养繁殖的培养基的熏蒸。

• 5. device and method for boundary fumigation of liquids, in particular culture media for the propagation of tissue culture [P] . 德国专利： DE3535183A1 . 1987-04-16

  机译： 液体，特别是用于组织培养繁殖的培养基的边界熏蒸的装置和方法

• 1. Elastic one-return boundary element method and hybrid elastic thin-slab propagator for strong-contrast media with sharp boundaries [J] . Zengxi Ge, Yi Luo, Yuyan Ma, 地震学报（英文版） . 2018,第002期
• 2. Quinclorac, a New Auxin-type Herbicide, Induces Embryogenic Callus and Somatic Embryogenesis in Plant Tissue Culture. [C] . Sang-Un Park, Peter J. Facchini, Chae Young-Am Annual Meeting of the Plant Growth Regulation Society of America . 2002
• 3. A higher-order direct boundary integral-displacement discontinuity method for fracture propagation in layered elastic media. [D] . Selcuk, Sakir. 1992
• 4. Relative loss of oncogenic potency of mouse leukemia virus (Gross) after prolonged propagation in tissue culture. [O] . L Gross, Y Dreyfuss 1978
• 5. Studies on the production of digitalis cardenolides by plant tissue culture. Part IV. Effects of mineral salts, initial pH and precursors on digitoxin formation by shoot-forming cultures of Digitalis purpurea L. grown in liquid media. [O] . Manabu HAGIMORI, Takashi MATSUMOTO, Yukiteru OBI 1983
• 6. Study of the Cytotoxic Effect of Staphylococcus Toxin on Tissue Culture. Report I. Selection of a Tissue Culture, Most Sensitive to the Action of Staphylococcus Toxin [R] . Belyaev, E. I. 1965

• 1. Condensed hyperelements method of non-vertical consistent boundaries for wave propagation analysis in irregular media [J] . Dorafshan S. ,Behnamfar F. ,Khamesipour A. . 地震工程与工程振动：英文版 . 2013,第4期
• 2. Phantom of Human Adipose Tissue and Studies of Light Propagation and Light Absorption for Parameterization and Evaluation of Noninvasive Optical Fat Measuring Devices [J] . Stefan Hartmann ,Matthias Moschall ,Oliver Schafer . 光学与光子学期刊（英文） . 2015,第2期
• 3. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 4. Overview of Research on Tissue Culture and Rapid Propagation Technology of Pholidota [J] . Miao ZHANG ,Long CHEN ,Hua ZHU . 农业生物技术:英文版 . 2021,第6期
• 5. 熏蒸牵引法应用于痹证型腰突症的临床疗效观察 [C] . 张建方 . 浙江省中西医结合学会骨伤科专业委员会第二十二届学术年会 . 2016
• 6. 生物熏蒸和热水消毒法替代甲基溴用于植物土传病害治理的研究 [A] . 李明社 . 2006