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HEPATITIS C VIRUS ANTIGEN POLYPEPTIDE, PRODUCTION THEREOF, AND DETECTION OF ANTIBODY

机译:丙型肝炎病毒病毒抗原多肽,其生产和抗体检测

摘要

A hepatitis C virus antigen polypeptide with a molecular weight of about 22 kd expressed from the structural gene domain of hepatitis C virus; a process for producing said polypeptide and/or related peptides characterized by introducing into a cultured cell strain an expression vector having a cDNA fragment of the structural gene domain of hepatitis C virus inserted thereinto and culturing the resultant transformed strain; and a method of detecting a hepatitis C virus antibody characterized by using the hepatitis C virus antigen polypeptide as an antigen to detect an antibody which is specific for the same.
机译:从丙型肝炎病毒的结构基因域表达的分子量约为22kd的丙型肝炎病毒抗原多肽;制备所述多肽和/或相关肽的方法,其特征在于将表达载体引入培养的细胞株中,所述表达载体具有插入的丙型肝炎病毒结构基因域的cDNA片段,并培养所得的转化株。一种检测丙型肝炎病毒抗体的方法,其特征在于使用丙型肝炎病毒抗原多肽作为抗原来检测对该抗体具有特异性的抗体。

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