SYNTHESIS AND USES OF SPIN LABELLED RIBONUCLEOSIDES AND RIBONUCLEOTIDES

摘要

Compounds of formulae (1), wherein R represents a CH3 or CD3 group; R1 represents an alkyl group; R2 represents an alkyl or aryl group; and N may be either an ?14N or 15¿N atom, with the proviso that when R represents CD¿3?, the methylene and vinyl hydrogen atoms of the six-membered ring are deuterium; and (2), wherein R and N are as defined above, X represents hydrogen or a mono-, di- or triphosphate group, or a phosphate ester derivative; and Y represents a purine or pyrimidine base, with the proviso that when R represents CD3, the methylene hydrogen atoms of the six-membered piperidine ring are hydrogen or deuterium. Processes for the preparation of these compounds are also described. The spin labelled nucleotide and nucleoside compounds are suitable for use as probes, for example, in protein structure and orientation studies.