Solid supports for nucleic acid hybridization assays and methods for covalently immobilizing oligonucleotides

摘要

Compositions and methods for covalently immobilizing an oligonucleotide onto a polymer-coated bead or similar structure are described. Specifically, the polymer-coated bead or similar structure possesses a large number of activatable moieties, preferably primary and secondary amines. An oligonucleotide is activated with a monofunctional or multi functional reagent, preferably the homotrifunctional reagent cyanuric chloride. Additionally, dichlorotriazine oligonucleotides and processes for activating oligonucleotides by treatment with cyanuric chloride are included in the present invention.