Detection assays having chromogenic and nonchromogenic, hypocromogenic, bathochromogenic or hypsochromogenic substrates

摘要

The invention relates to enzyme detection assay compositions and methods which have chromogenic and nonchromogenic, hypochromogenic, bathochromogenic or hypsochromogenic substrates. By using both chromogenic and nonchromogenic, hypochromogenic, bathochromogenic or hypsochromogenic substrates assay sensitivity can be controlled and the need for sample predilution eliminated. In one embodiment of the invention an assay for the estimation of serum cholinesterase activity is provided.