Method for highly sensitive determination of NADH using NADH kinase

摘要

The present invention relates to a method for determining a slight amount of NADH or XTP (wherein X is A, V, G, C, I, dT, dA, dV, dG, dC or dI) which is present in a solution, with high sensitivity by the use of an NADH kinase specific for NADH by utilizing a cycling reaction, and this method permits highly sensitive determination of NADH without any influence of NAD⁺ and hence is useful for diagnoses of diseases and the like.