STRAIN OF BACTERIA BACILLUS ALVEI - PRODUCER OF RESTRICTION ENDONUCLEASE BAV BII

摘要

use: u0431u0438u043eu0442u0435u0445u043du043eu043bu043eu0433u0438u00a0 and mo u043bu0435u043au0443u043bu00a0u0440u043du043e genetic work and u0433u0435u043du043du043eu0438u043d - u0436u0435u043du0435u0440u043du044bu0445 u0438u0441u0441u043bu0435u0434u043eu0432u0430u043du0438u00a0u0445. the essence of the u0438u0437u043eu0431u0440u0435u0442u0435u043du0438u00a0: biomass in alvei BKM in 674 grow under aerobic u0443u0441u043bu043eu0432u0438u00a0u0445 in breeding environment containing sources of carbon, nitrogen and mineral salts, to late logarithmic phase of growth, the target product of biomass u0432u044bu0434u0435u043bu00a0u044eu0442 obtained by known methods.the biomass of strain of bacteria u0432.alvei BKM in 674 contains a restriction u044du043du0434u043eu043du0443u043au043bu0435u0430u0437u0443 Bav in ii, u0443u0437u043du0430u044eu0449u0443u044e and u0440u0430u0441u0449u0435u043fu043bu00a0u044eu0449u0443u044e u043du0443u043au043bu0435u043eu0442u0438u0434u043du0443u044e dna sequence 5. g CNCC... 3, in quantities sufficient u0434u043bu00a0 u043fu043eu043bu0443u0447u0435u043du0438u00a0 u0440u0435u0441u0442u0440u0438u043au0442u0430u0437u044b in u043fu0440u0435u043fu0430u0440u0430u0442u0438u0432u043du044bu0445 scale: the u0440u0435u0441u0442u0440u0438u043au0442u0430u0437u044b Bav BII biomass 3 - 4 x 10 iu / g of crude microbial mass.u0434u043bu00a0 u0440u0435u0441u0442u0440u0438u043au0442u0430u0437u044b Asu i, u0438u0437u043eu0448u0438u0437u043eu043cu0435u0440u0430 restriction enzyme Bav BII enzyme content in biomass, and a purified enzyme preparation are not specified. a pure substance u0440u0435u0441u0442u0440u0438u043au0442u0430u0437u044b Bav BII u0441u043eu0441u0442u0430u0432u043bu00a0u0435u0442 8 to 12 x 103u0435u0434 in 1 g of crude biomass. u0440u0435u0441u0442u0440u0438u043au0442u0430u0437u044b Bav BII contains impurities u0444u043eu0441u0444u0430u0442u0430u0437, unspecified and u0434u043eu043du0443u043au043bu0435u0430u0437 and u044du043au0437u043eu043du0443u043au043bu0435u0430u0437. v fe