STRAIN OF BACTERIA BACILLUS COAGULANTS - PRODUCER OF RESTRICTION ENDONUCLEASE BCO AI

摘要

use: u0431u0438u043eu0442u0435u0445u043du043eu043bu043eu0433u0438u00a0 and in practice u043cu043eu043bu0435u043au0443u043bu00a0u0440u043du043e genetic and u0433u0435u043du043du043eu0438u043d - u0436u0435u043du0435u0440u043du044bu0445 works. the essence of the u0438u0437u043eu0431u0440u0435u0442u0435u043du0438u00a0: a producer B.coagulans cmes in that grow with aeration in the breeding environment containing sources of carbon, nitrogen and mineral salts, until later. u0433u0430u0440u0438u0444u043cu0438u0447u0435u0441u043au043eu0439 phases of growth, the target product of biomass u0432u044bu0434u0435u043bu00a0u044eu0442 obtained by known methods. the biomass of b.coagulans cmes in that contains a new site - specific u044du043du0434u043eu043du0443u043au043bu0435u0430u0437u0443 BcoAl, u0443u0437u043du0430u044eu0449u0443u044e and u0440u0430u0441u0449u0435u043fu043bu00a0u044eu0449u0443u044e sequence of nam to 5 - a - g - t - g 3, in quantities sufficient u0434u043bu00a0 floor u0447u0435u043du0438u00a0 u0440u0435u0441u0442u0440u0438u043au0442u0430u0437u044b in u043fu0440u0435u043fu0430u0440u0430u0442u0438u0432u043du044bu0445 quantities, contents u0440u0435u0441u0442u0440u0438u043au0442u0430u0437u044b BcoAl biomass 6000 - 8000 iu / g raw biomass. u0434u043bu00a0 u0438u0437u043eu0448u0438u0437u043eu043cu0435u0440u0430 BcoAl, u0440u0435u0441u0442u0440u0438u043au0442u0430u0437u044b PmaCl, exit with purified enzyme 20 units of 1 g wet weight biomass.a pure substance restriction enzyme BcoAl u0441u043eu0441u0442u0430u0432u043bu00a0u0435u0442 700 to 1200 iu. activity of 1 g of crude biomass that in 35 - 60 times higher than the reported figures u0434u043bu00a0 u0440u0435u0441u0442u0440u0438u043au0442u0430u0437u044b PmaCl. u044du043du0434u043eu043du0443u043au043bu0435u0430u0437u0430 restriction BcoAl contains impurities u0444u043eu0441u0444u0430u0442u0430u0437, u044du043au0437u043eu043du0443u043au043bu0435u0430u0437 and unspecified u044du043du0434u043eu043du0443u043au043bu0435 - az. oW yu