Alpha-glucosidase gene from Candida tsukubaensis cloned and expressed in Saccharomyces cerevisiae

摘要

A gene (Figure 5) encoding an amylolytic enzyme (alpha-glucosidase) from Candida tsukubaensis has been cloned, sequenced, and expressed in Saccharomyces cerevisiae. The cloned material is present on the plasmid pGA8701 (which has been deposited as S. cerevisiae AH22(pGA8701) in the National Collection of Yeast Cultures with the number NCYC 2345). The enzyme is heat labile and S. cerevisiae strains expressing the enzyme are suitable for use in the production of low carbohydrate beers. The cloned DNA also contains a strong promoter useful for directing the expression of other heterologous genes in S. cerevisiae. IMAGE