Cloning of Glucoamylase Gene from Aspergillus niger and its Expression in Saccharomyces cerevisiae W303-1B

摘要

To further research the heterologous expression of glucoamylase gene, Saccharomyces cerevisiae W303-1B as host strain to express the glucoamylase gene from Aspergillus niger was studied. Glucoamylase gene GAL was cloned from A. niger and analyzed. The results showed the GAL had an open reading frame of 1923 bp that encoded a protein of 640 amino acids. The glucoamylase-producing engineering strain W303-1B/pYPGE15-GAL was constructed by cloning the GAL gene into S. cerevisiae expression vector pYPGE15 and transforming it into S. cerevisiae W303-1B. The results reveal that the recombinant glucoamylase is expressed and secreted correctly in engineering strain W303-1B/pYPGE15-GAL, and its enzyme activity in the medium reaches 212.9 U/ml.