首页> 外国专利> DNA probes for the detection of Haemophilus Ducreyi and method of use

DNA probes for the detection of Haemophilus Ducreyi and method of use

机译:用于检测Ducreyi嗜血杆菌的DNA探针及其使用方法

摘要

This invention provides DNA probes which are useful for the detection of Haemophilus ducreyi. This invention also provides a method of detecting in a subject the presence of H. ducreyi which comprises obtaining a suitable sample from the subject, denaturing the DNA in the sample so as to produce single stranded DNA molecules, contacting the single stranded DNA molecules so obtained with a single stranded oligonucleotide probe, under suitable conditions which permit hybridization of complementary single stranded molecules, and detecting the presence of hybridized molecules, whereby the presence of H. ducreyi in the subject is detected. This invention further comprises a kit which is useful for the detection of H. ducreyi.
机译:本发明提供了可用于检测Ducreyi嗜血杆菌的DNA探针。本发明还提供了一种在受试者中检测杜克氏杆菌存在的方法,该方法包括从受试者获得合适的样品,使样品中的DNA变性以产生单链DNA分子,使如此获得的单链DNA分子接触。在允许互补单链分子杂交并检测杂交分子存在的合适条件下,用单链寡核苷酸探针用单链寡核苷酸探针进行检测,从而检测对象中杜克氏杆菌的存在。本发明进一步包括可用于检测杜克氏杆菌的试剂盒。

著录项

相似文献

  • 专利
  • 外文文献
  • 中文文献
获取专利

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号