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Methods and reagents for the detection of herpes simplex virus, treponema pallidum, and haemophilus ducreyi
Methods and reagents for the detection of herpes simplex virus, treponema pallidum, and haemophilus ducreyi
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机译:用于检测单纯疱疹病毒,梅毒螺旋体和杜克雷嗜血杆菌的方法和试剂
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摘要
The present invention provides methods and reagents for the detection and identification of four causative agents of genital ulcerations, herpes simplex virus (HSV) types 1 and 2, Treponema pallidum, and Haemophilus ducreyi. The methods use sequence-specific primers which enable the simultaneous polymeric chain reaction amplification of genomic nucleic acid sequences from HSV types 1 and 2, T. pallidum, and H. ducreyi. Following amplification, sequence-specific oligonucleotide probes are used to detect and distinguish HSV type 1 and 2, T. pallidum, and H. ducreyi nucleic acid.
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