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Efficient process for isolating insoluble heterologous protein using non- ionic detergents
Efficient process for isolating insoluble heterologous protein using non- ionic detergents
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机译:使用非离子型去污剂分离不溶性异源蛋白的有效方法
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摘要
The present invention relates to the isolation and restoration of biological activity to inactive proteins, that is solubilizing, renaturing and restoring activity to proteins which have been partially denatured or inactivated, e.g. during their synthesis in a host cell, such as E. coli, or during isolation. In particular this invention relates to both a means for extracting insoluble eukaryotic proteins from bacteria and to an efficient process for producing active chymosin from an insoluble chymosin precursor isolated from genetically engineered bacteria.
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