Monoclonal antibodies specific for protease nexin-1 and puriciation of protease nexin-1 using monoclonal antibodies

摘要

Monoclonal antibodies having a specificity for protease nexin- 1 are described. Preferably an antibody has specificity to the reactive protease-binding site. The antibody is used in the affinity purification of PN-1 to produce large quantities of substantially pure PN-1, free of other biologically active molecules. Over a 2000-fold purification of a crude preparation of PN-1 may be obtained. The affinity-purified PN-1 is suitable for use in pharmacological preparations used in the treatment of neurological disease associated with serine protease-mediated inhibition of regenerative processes.