DETERMINATION OF FINE AMOUNT OF REDUCTION TYPE AND OXIDATION TYPE NICOTINAMIDE ADENINE DINUCLEOTIDE OR REDUCTION TYPE AND OXIDATION TYPE NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE

摘要

PURPOSE: To provide a method for determining the fine amount of a reduction type or oxidation type nicotinamide denine dinucleotide or a reduction type or oxidation type nicotinamide adenined dinucleotide phosphate. ;CONSTITUTION: A method for determining the fine amount of NAD(P)H or NAD(P) or an enzyme and an substrate or a product which are related thereto is characterized by reacting a specimen containing the NAD(P)H or NAD(P) with resazurin or p-iodonitrotetrazolium violet (INT-V) in the presence of a catalyst, amplifying the absorption of the reaction product in the visible region light with the substrate of a NAD(P)-NAD(P)H cycling conjugated with the reaction product and with an enzyme system and subsequently measuring the change of the absorption.;COPYRIGHT: (C)1993,JPO&Japio