ASSAY OF REDUCING TYPE NICOTINAMIDE ADENINE DINUCLEOTIDE AND REDUCING TYPE NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE

摘要

PURPOSE:To assay reducing type nicotinamide adenine dinucleotide phosphate 'NAD(P)H' handily with a high sensitivity, by making reducing type nicotinamide adenine dinucleotide or NAD(P)H react with resazurin to measure the absorbance at the wavelength near the maximization of absorption. CONSTITUTION:NAD(P)H is made to react with resarzurin using a catalyst such as diaphorase to from resorufine. The resazurin is a blue substance having the maximization of absorption near 600nm on the slight alkaline side while the resorufine is a red substance having the maximization of absorption near 570nm. Therefore, the assay of NAD(P)H can be done by measuring the absorbance at the wavelength near the respective maximization of absorption. In the measurement of the absorbance, for example, a microplate type 1-wavelength spectrometer is used.