METHOD FOR RAPIDLY TRANSFORMING GRAM-NEGATIVE BACTERIUM AND EXPERIMENT KID FOR PERFORMING THE SAME

摘要

PURPOSE: To provide a method for rapidly transforming a gram-negative bacterium, especially Escherichia coli, and to provide an experimental kid for its performance. ;CONSTITUTION: Escherichia coli is brought into contact with a solution containing a desired plasmid DNA, a polyethylene glycol aqueous solution containing alkali metal ions, and a multiplication medium in this order to perform its phenotypic expression. An Escherichia-receiving container A, a plasmid DNA aqueous solution-receiving container B, a polyethylene glycol aqueous solution-receiving container C, and a multiplication medium-receiving container D are received in a container 1 to form the experimental kid. If necessary, an agar medium for detecting the transformation may be received in the experimental kid. Thereby, the phenotypic expression rapidly proceeds. A freezing temperature operation and a centrifugal operation are not required. The transformation can be carried out even for bacteria not placed in its logarithmic growth period.;COPYRIGHT: (C)1993,JPO&Japio